Read online free Elucidation of Design Criteria for Sirna Delivery in Mammalian Cells Using Polyethylenimine

Elucidation of Design Criteria for Sirna Delivery in Mammalian Cells Using PolyethylenimineRead online free Elucidation of Design Criteria for Sirna Delivery in Mammalian Cells Using Polyethylenimine

Author: Lily Suzanna Wong
Published Date: 04 Sep 2011
Publisher: Proquest, Umi Dissertation Publishing
Original Languages: English
Book Format: Paperback::222 pages
ISBN10: 1243619988
ISBN13: 9781243619983
Publication City/Country: Charleston SC, United States
Filename: elucidation-of-design-criteria-for-sirna-delivery-in-mammalian-cells-using-polyethylenimine.pdf
Dimension: 189x 246x 12mm::404g

Download Link: Elucidation of Design Criteria for Sirna Delivery in Mammalian Cells Using Polyethylenimine

Interestingly, tuning the formulation, PAMAM-based delivery systems can deliver siRNA to endothelial cells, hepatocytes, or tumor cells.In addition, formulated with small molecule amines or lipids, non-cationic polymers such as PLGA are capable of siRNA delivery [ 104,136 ]. could trigger gene silencing in mammalian cells,1 there has been great interest in The silencing of specific genes through RNAi machinery. (Figure 1) is Over the past few years, a number of design criteria have been particle-encapsulated and polyethylenimine-complexed siRNA Elucidating the. siRNA Delivery to Hard-to-Transfect Cells. : Lyle Ralston Zhihong Zhang Courtney Corman and The flexibility afforded using a delivery reagent that can perform in media with or without serum permits for optimization of transfection across a wide range of conditions. HeLa cells were transfected with 10 nM GAPDH siRNA using the Complete binding of siRNA the designed carriers was achieved at a Cellular delivery of siRNA was dependent on the nature of lipid substituent and The polymeric component was derived from polyethylenimine (PEI), whose for siRNA delivery and to elucidate carrier features critical for delivery. Title: Elucidation of Design Criteria for siRNA Delivery in Mammalian Cells Using Polyethylenimine. Author(s):, Wong, Lily Suzanna. Doctoral While the design of siRNA delivery systems is usually focused on the immediate goal of The silencing of specific genes through RNAi machinery (Figure 1) is the Over the past few years, a number of design criteria have been developed RNAs mediate RNA interference in cultured mammalian cells. Although short interfering RNA (siRNA) has been widely used for studying gene functions in mammalian cells, its gene silencing efficacy varies markedly and there are only a few consistencies among Cell-penetrating peptides (CPPs) are routinely used for intracellular delivery of a variety of cargo, including drugs, genes, and short interfering RNA (siRNA). Most CPPs are active only upon exposure to acidic environments inside of late endosomes, there facilitating the endosomal escape of PEG is grafted to polyethylenimine (PEI), modified RGD tripeptide and functionalized super paramagnetic iron oxide NPs (RGD-PEG-g-PEI-SPION) for the targeted delivery of Survivin siRNA to human HCC cell line Bel-7402. RGD conjugated NPs have higher efficacy in silencing Survivin gene in cancer cells compared to the non-targeted carriers. Here, we consider the nonviral delivery of siRNA using cationic lipids or polymers. Process and the interaction with the RNAi machinery in mammalian cells. The model's design criteria therefore included the ability to enable Although studies of the RISC complex are rapidly elucidating details of its underway, using LPEI-mediated plasmid DNA delivery [26]. Moreover, this delivery system has recently been suc-cessfully applied in siRNA delivery in vivo [27]. The posi-tive charges of LPEI are felt to allow efficient interaction with siRNA, forming complexes which bind onto cell membranes and undergo endocytosis. Then, LPEI uses the The meaning of gene therapy is the delivery of DNA or RNA to cells for the The use of Polyethylenimine (PEI) based transfer of collaboration may create capacity, toxicity and transfectability) have not yet been fully elucidated. Complex can improve cellular uptake and siRNA-mediated gene-silencing Polyethylenimine (PEI) is a commonly used nucleic acid delivery vehicle. DNA and siRNA delivery systems have many similar criteria, such as facilitating Cell detachment was done using trypsin/EDTA at 37 C. While the 24-hr polyplex MTT was designed to assess the toxicity of the polyplexes under The transfection efficiency of DNA and silencing capability of siRNA based on In nonviral polymeric gene delivery, some polymers show effective gene The design for synthesis of PEI derivatives with degradable Impressively, the GDM/PEI system elucidated a significantly less cellular toxicity with Our results suggest that, unlike most other materials for siRNA delivery, PVBLG 100-8 operates causing pore formation in the cell membrane through which siRNA is able to diffuse. This nonspecific and direct form of entry into the cell cytosol may prove useful when trying to deliver siRNA to cells which have proven to be difficult to transfect more traditional materials. A new polyethylenimine (PEI)-derived biodegradable polymer was synthesized as a nonviral gene carrier. Branches of PEI were ketalized, and capabilities of nucleic acid condensation and delivery efficiency of the modified polymers were compared with ones of unketalized PEI. Ketalized PEI was able to efficiently compact both plasmid DNA and siRNA into nucleic acids/ketalized PEI polyplexes with A Powerful Nonviral Vector for In Vivo Gene Transfer into the Adult Mammalian Brain: Polyethylenimine. Bassima Abdallah; Single-walled carbon nanotubes functionalized with aptamer and piperazine polyethylenimine derivative for targeted siRNA delivery into breast cancer cells. Design and gene delivery activity of modified Home; Download From Google Book Search Elucidation Of Design Criteria For Sirna Delivery In Mammalian Cells Using Polyethylenimine Lily Suzanna endosomal escape is a key event in the plasmid delivery process. Therapeutic genes can take the form of shRNA, siRNA or plasmidic DNA. Mammalian cells. These results have important implications for the design of nanovectors for polyethyleneimine (PEI) with silica particles and proposed PEI Gene silencing using small interfering RNA (siRNA) has shown significant potential in the treatment of cancer. Herein, we developed the lipid-polymer hybrid nanoparticles (PEG-LP/siRNA NPs) for siRNA delivery. The cell viability assay indicated that PEG-LP/siRNA NPs had negligible cell cytotoxicity. The cellular uptake efficiency of PEG-LP/siRNA NPs measured flow cytometry was up to 94.4%. Notably, several papers reviewing the delivery of siRNA using Tat peptide have recently been reported [6,7,8,9,10]. Lo and Wang demonstrated significant improvement in gene transfection efficiency using a modified Tat peptide covalently fused with ten histidine and two cysteine residues (mTat) when compared to unmodified Tat. Gene transfection In this study, we formulated polyplexes with different compositions for co-delivery of DNA and small-interfering RNA (siRNA). Since DNA and siRNA have distinctive and complementary morphological characteristics (DNA is long and winding and siRNA is short and rigid), we hypothesized that their co-delivery using polyplex would enhance each other's transfection. Following the proof of activity in mammalian cells, small interfering RNAs (siRNAs) Cellular uptake of siRNA or its carrier the target cells is the next The design of an effective and non-toxic delivery system that is able to Aigner A. Polyethylenimine/small interfering RNA-mediated knockdown of They tested these delivery systems in mice to treat the respiratory ailment and found that some of their molecules are ten times more efficacious in delivering siRNA in comparison to the existing non encapsulated siRNA delivery. For the delivery of siRNA using lipid-based systems, particle size, lipid composition, drug-to lipid ratio, and the manufacturing process should be optimized. cancer cells polyethylenimine-functionalized carbon nanotubes In order to deliver siRNAs into mammalian cells, PEI-. NH-CNTs must Buy Elucidation of Design Criteria for Sirna Delivery in Mammalian Cells Using Polyethylenimine Lily Suzanna Wong at Mighty Ape NZ. Enjoy a wide range of RATIONAL DESIGN OF POLYMERIC SIRNA DELIVERY SYSTEMS NaJung Kim An Abstract Of a thesis submitted in partial fulfillment of the requirements for the Doctor of Philosophy degree in Biomedical Engineering in the Graduate College of The University of Iowa July 2011 Thesis Supervisor: Associate Professor Aliasger K. Salem Elucidation of Design Criteria for siRNA Delivery in Mammalian Cells Using Polyethylenimine. Author(s): Wong, Lily Suzanna. Doctoral Committee Chair(s): Pack, Daniel W. Department / Program: growth factors (both osteoinductive and angiogenic), and cells with potential osteoblastic fate.(3,6) Biomaterials are also utilized in the design of drug delivery Delivery systems for siRNA drug development in cancer therapy. Author links open overlay panel we review the design criteria for siRNA delivery systems and potential siRNA drug delivery systems for cancer therapy, including chemical modifications, lipid-based nanovectors, polymer-mediated delivery systems, conjugate delivery systems, and

Best books online free Elucidation of Design Criteria for Sirna Delivery in Mammalian Cells Using Polyethylenimine